May 10th, 2014
•This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.
Related Videos
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching (Video) | JoVE
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases (Video) | JoVE
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry (Video) | JoVE
Crystallization of Membrane Proteins in Lipidic Mesophases (Video) | JoVE
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry (Video) | JoVE
Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT) (Video) | JoVE
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases (Video) | JoVE
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins (Video) | JoVE
Imaging Plasma Membrane Deformations With pTIRFM (Video) | JoVE
Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved