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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

DOI :

10.3791/58582-v

December 1st, 2018

December 1st, 2018

10,966 Views

1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

This protocol describes a fluorescence fluctuation spectroscopy-based approach to investigate interactions among proteins mediating cell-cell interactions, i.e. proteins localized in cell junctions, directly in living cells. We provide detailed guidelines on instrument calibration, data acquisition and analysis, including corrections to possible artefact sources.

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