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Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

DOI :

10.3791/57837-v

June 15th, 2018

June 15th, 2018

8,128 Views

1Department of Experimental Plant Biology, Faculty of Science, Charles University

Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.

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Plasma Membrane Partitioning

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