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Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

DOI :

10.3791/61823-v

5:56 min

November 12th, 2020

November 12th, 2020

2,268 Views

1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

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Spot Variation Fluorescence Correlation Spectroscopy

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