November 12th, 2020
•This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.
Tags
Related Videos
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved