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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

DOI :

10.3791/51114-v

January 17th, 2014

January 17th, 2014

7,526 Views

1Inserm U1019 - CNRS UMR 8024, Institut Pasteur de Lille, Université de Lille

Here, we describe a phenotypic assay applicable to the High-throughput/High-content screens of small-interfering synthetic RNA (siRNA), chemical compound, and Mycobacterium tuberculosis mutant libraries. This method relies on the detection of fluorescently labeled Mycobacterium tuberculosis within fluorescently labeled host cell using automated confocal microscopy.

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