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Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

DOI :

10.3791/52851-v

11:44 min

May 13th, 2015

May 13th, 2015

9,373 Views

1Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS, Université Montpellier

Coxiella burnetii is an obligate intracellular Gram-negative bacterium responsible for the zoonotic disease Q fever. Here we describe methods for the generation of Coxiella fluorescent transposon mutants as well as the automated identification and analysis of the resulting internalization, replication and cytotoxic phenotypes.

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Generation

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