JoVE Logo
Faculty Resource Center

Sign In

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI :

10.3791/51151-v

9:27 min

January 17th, 2014

January 17th, 2014

7,174 Views

1School of Molecular Bioscience, University of Sydney, 2Department of Medicine, Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science, University of Melbourne

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Tags

Methodology

-- Views

Related Videos

article

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

article

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

article

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

article

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

article

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

article

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

article

Assays for the Identification of Novel Antivirals against Bluetongue Virus

article

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

article

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

article

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved