JoVE Logo
Faculty Resource Center

Sign In

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

DOI :

10.3791/51522-v

May 15th, 2014

May 15th, 2014

11,185 Views

1Department of Microbiology, Boston University School of Medicine

We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.

Tags

Vaccinia Reporter Viruses

-- Views

Related Videos

article

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

article

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

article

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

article

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

article

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

article

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

article

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

article

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

article

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

article

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved