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A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

DOI :

10.3791/54171-v

9:16 min

October 30th, 2016

October 30th, 2016

10,693 Views

1Center for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University

Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.

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Mutant Vaccinia Virus Vectors

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