JoVE Logo
Faculty Resource Center

Sign In

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

DOI :

10.3791/54171-v

9:16 min

October 30th, 2016

October 30th, 2016

10,722 Views

1Center for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University

Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.

Tags

Mutant Vaccinia Virus Vectors

-- Views

Related Videos

article

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

article

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

article

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

article

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

article

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

article

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

article

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

article

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

article

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

article

Assays for the Identification of Novel Antivirals against Bluetongue Virus

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved