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Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

DOI :

10.3791/51112-v

November 6th, 2013

November 6th, 2013

23,329 Views

1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface proteins.

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