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Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

DOI :

10.3791/51206-v

13:52 min

March 19th, 2014

March 19th, 2014

22,940 Views

1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

Kindlins are fundamental to cell adhesion through integrins but studies of them have been hampered by the difficulty encountered in expressing them recombinantly in bacterial hosts. We describe here methods for their efficient production in baculovirus-infected insect cells.

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Recombinant

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