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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

DOI :

10.3791/58283-v

August 20th, 2018

August 20th, 2018

10,808 Views

1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences, North Carolina State University

Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.

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