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JoVE Journal

Neuroscience

This content is Open Access.

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

37.1K Views

09:52 min

February 25th, 2014

February 25th, 2014

37,115 Views

0:05

Title

1:41

Microdissection of Mouse Brain

4:13

SVZ Tissue Dissociation

5:43

DG Tissue Dissociation

7:36

Cell Plating for Monolayer and Neurosphere Culture Generation

8:17

Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

9:21

Conclusion

Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.

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