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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

DOI :

10.3791/51265-v

March 14th, 2014

March 14th, 2014

12,690 Views

1Department of Biology, Technion - Israel Institute of Technology

Many mRNAs encoding mitochondrial proteins are associated with the mitochondria outer membrane. We describe a subcellular fractionation procedure aimed at isolation of yeast mitochondria with its associated mRNAs and ribosomes. This protocol can be applied to cells grown under diverse conditions in order to reveal mechanisms of mRNA localization and localized translation near the mitochondria.

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Isolation

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