August 22nd, 2017
•This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.
Tags
Related Videos
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Ubiquitin Chain Analysis by Parallel Reaction Monitoring
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved