February 25th, 2011
•A major impediment to biochemical analyses of ribosomes containing nascent peptidyl-tRNAs has been the presence of other ribosomes in the same samples, ribosomes not involved in the translation of the specific mRNA sequence being analyzed. We developed a simple methodology to purify, exclusively, the ribosomes containing the nascent peptidyl-tRNA of interest.
Tags
Related Videos
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
Isolation and Characterization of RNA-Containing Exosomes
Chromatographic Purification of Highly Active Yeast Ribosomes
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved