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Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

DOI :

10.3791/51418-v

March 25th, 2014

March 25th, 2014

14,533 Views

1Department of Biochemistry, Purdue University

Drosophila tissues often contain a heterogeneous mixture of cell types. To examine gene expression in specific cell types from a particular tissue, nuclei can be genetically tagged and subsequently isolated using an affinity-based approach. Isolated nuclei can be used for downstream applications such as gene expression analysis and chromatin immunoprecipitation.

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Affinity based Isolation

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