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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

DOI :

10.3791/1904-v

10:34 min

April 14th, 2010

April 14th, 2010

15,148 Views

1Gene Expression Division, Bio-Rad Laboratories

This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.

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Automated Cell Counter

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