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In vitro Reconstitution of the Active T. castaneum Telomerase

DOI :

10.3791/2799-v

July 14th, 2011

July 14th, 2011

11,568 Views

1Gene Expression and Regulation, The Wistar Institute, University of Pennsylvania

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more than a decade. Here, we present methods for the isolation of the recombinant Tribolium castaneum TERT (TcTERT) and the reconstitution of the active T. castaneum telomerase ribonucleoprotein (RNP) complex in vitro.

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Telomerase

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