JoVE Logo
Faculty Resource Center

Sign In

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

DOI :

10.3791/51420-v

14:53 min

May 13th, 2014

May 13th, 2014

11,971 Views

1Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK

We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.

Tags

RNA Synthesis

-- Views

Related Videos

article

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

article

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

article

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

article

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

article

Single-cell Microinjection for Cell Communication Analysis

article

Measurement of Heme Synthesis Levels in Mammalian Cells

article

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

article

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

article

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

article

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved