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Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

DOI :

10.3791/51628-v

June 14th, 2014

June 14th, 2014

27,157 Views

1Department of Reproduction and Development, Erasmus MC - University Medical Center

Fluorescent in situ hybridization (FISH) allows the detection of nucleic acids in their native environment within cells. We here describe a protocol for the combined, simultaneous detection of RNA and DNA by means of FISH, which can be used to study X chromosome inactivation in mouse embryonic stem cells.

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Keywords DNA FISH

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