JoVE Logo
Faculty Resource Center

Sign In

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

DOI :

10.3791/51628-v

•

June 14th, 2014

June 14th, 2014

•
27,157 Views

1Department of Reproduction and Development, Erasmus MC - University Medical Center

Fluorescent in situ hybridization (FISH) allows the detection of nucleic acids in their native environment within cells. We here describe a protocol for the combined, simultaneous detection of RNA and DNA by means of FISH, which can be used to study X chromosome inactivation in mouse embryonic stem cells.

Tags

DNA RNA FISH

-- Views

Related Videos

article

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

article

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

article

Isolation and Derivation of Mouse Embryonic Germinal Cells

article

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

article

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

article

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

article

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

article

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

article

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

article

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved