JoVE Logo
Faculty Resource Center

Sign In

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

DOI :

10.3791/59449-v

May 22nd, 2019

May 22nd, 2019

6,147 Views

1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, 2Department of Neuroscience, University of Virginia School of Medicine

Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.

-- Views

Related Videos

article

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

article

Exercise Test for Evaluation of the Functional Efficacy of the Pig Cardiovascular System

article

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

article

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

article

Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains

article

Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

article

Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

article

Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved