Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

31.3K Views

12:53 min

July 6th, 2014

DOI :

10.3791/51656-v

July 6th, 2014


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SILAC

Chapters in this video

0:05

Title

1:49

Harvesting of SILAC-labeled Cell Lysates

3:39

Binding Lysate to Anti-GFP Beads

4:45

Preparation of Samples for MS Analysis

6:11

Data Analysis I: Understanding the Results and Removal of Low-confidence Identifications

7:36

Data Analysis II: Selecting High Confidence Interactions for Further Study

8:58

Data Analysis III: Merging Replica Datasets

10:59

Results: Identification of Translation Initiation Factor – Binding Proteins

12:22

Conclusion

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