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siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

DOI :

10.3791/51572-v

May 24th, 2014

May 24th, 2014

11,260 Views

1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

Here, we describe a methodology to perform a targeted siRNA “ubiquitome” screen to identify novel ubiquitin and ubiquitin-like regulators of the HIF1A-mediated cellular response to hypoxia.  This can be adapted to any biological pathway where a robust read out of reporter activity is available.

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