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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

DOI :

10.3791/52255-v

8:38 min

March 3rd, 2015

March 3rd, 2015

12,901 Views

1Département de Biologie, Institut de biologie intégrative et des systémes & PROTEO, Université Laval

Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

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Protein protein Interaction Screening

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