JoVE Logo
Faculty Resource Center

Sign In

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

DOI :

10.3791/1963-v

June 12th, 2010

June 12th, 2010

19,325 Views

1Dept. Of Cell Biology and Molecular Genetics, University of Maryland

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Tags

Bimolecular Fluorescence Complementation

-- Views

Related Videos

article

Gene-gun Transfection of Hippocampal Neurons

article

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

article

Bimolecular Fluorescence Complementation

article

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

article

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

article

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

article

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

article

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

article

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

article

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved