Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
21.4K Views
•
13:58 min
•
June 25th, 2014
DOI :
June 25th, 2014
•1Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 2Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 3Department of Ophthalmology, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
Chapters in this video
0:06
Title
2:01
Dorsal Skin for Immunostaining and Melanin Imaging
5:40
Foot Skin for the Analysis of Hair Follicle Patterning
6:42
Foot Skin for Visualizing Sebaceous Glands
7:23
Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
8:54
Imaging Hair Follicle Orientation
9:52
Imaging and Tracing Axon Arbors
10:55
Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
12:51
Conclusion
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