JoVE Logo
Faculty Resource Center

Sign In

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

DOI :

10.3791/51810-v

June 8th, 2014

June 8th, 2014

11,471 Views

1Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, 2Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children - Northern California, 3Department of Dermatology, University of California, Davis

Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.

Tags

In Vivo Gene Transfer

-- Views

Related Videos

article

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia

article

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

article

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

article

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

article

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model

article

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind

article

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

article

A Novel Application of Musculoskeletal Ultrasound Imaging

article

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

article

In vivo 19F MRI for Cell Tracking

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved