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Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

DOI :

10.3791/54205-v

July 28th, 2016

July 28th, 2016

7,944 Views

1Princess Margaret Cancer Centre, 2Department of Medical Biophysics, University of Toronto, 3Techna Institute, University Health Network

The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

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