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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

DOI :

10.3791/51824-v

September 5th, 2014

September 5th, 2014

12,053 Views

1Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Geology, University of Illinois at Urbana-Champaign, 3Department of Microbiology, University of Illinois at Urbana-Champaign

An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

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