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Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

DOI :

10.3791/57087-v

March 6th, 2018

March 6th, 2018

10,522 Views

1Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS, Institut Pasteur, 2UTechS Photonic BioImaging (Imagopole) Citech, Institut Pasteur

The overall goal of this methodology is to give the optimal experimental conditions from sample preparation to image acquisition and reconstruction in order to perform 2D dual color dSTORM images of microtubules and intermediate filaments in fixed cells

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2 dimensional Direct Stochastic Optical Reconstruction Microscopy

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