Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

9.7K Views

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08:33 min

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December 4th, 2014

DOI :

10.3791/51853-v

December 4th, 2014

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Soumya Narayan¹, Charlee McLean², Akira Sawa¹, Sandra Y. Lin³, Narayan Rai², MariaMananita S. Hipolito², Nicola Cascella⁴, John J.I. Nurnberger, Jr.⁵, Koko Ishizuka¹, Evaristus A. Nwulia²

¹Department of Psychiatry, Johns Hopkins University, ²Department of Psychiatry and Behavioral Sciences, Howard University, ³Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University, ⁴Department of Psychiatry, Sheppard Pratt Hospital, ⁵Department of Psychiatry, Indiana University

Summary

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Keywords Olfactory Neurons

Laser capture Microdissection

Bipolar Disorder

Lithium Carbonate

Neuropsychiatric Disorders

Olfactory Epithelium

Treatment Response

Chapters in this video

0:05

Title

1:49

Laser-Capture Microdissection

3:48

Isolation of Total RNA and cDNA Synthesis

5:50

Real Time PCR Quantification

7:14

Reults: GSK-3β Expression in OE Neurons

7:53

Conclusion

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