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Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

DOI :

10.3791/51896-v

September 3rd, 2014

September 3rd, 2014

30,501 Views

1Department of Pharmacology and Toxicology, University of Toronto

This article details the enrichment of proteins associated with the synaptic plasma membrane by ultracentrifugation on a discontinuous sucrose gradient. The subsequent preparation of post-synaptic density proteins is also described. Protein preparations are suitable for western blotting or 2D DIGE analysis.

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Synaptic Plasma Membrane

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