An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses
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07:03 min
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March 28th, 2025
DOI :
March 28th, 2025
•Lauryn Ruegg1,2, Laura B. James-Allan1,2, Gabriella DiBernardo1,2, Christopher Ochoa1,2,3, Yan-Ruide Li4,5, Lili Yang2,3,4,5,6,7, Sanaz Memarzadeh1,2,3,7,8,9
1Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California, Los Angeles, 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 3Molecular Biology Institute, University of California, Los Angeles, 4Department of Bioengineering, University of California, Los Angeles, 5Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 6Parker Institute for Cancer Immunotherapy, University of California, Los Angeles, 7The Jonsson Comprehensive Cancer Center, University of California Los Angeles, 8The VA Greater Los Angeles Healthcare System, 9Department of Medical and Molecular Pharmacology, David Geffen School of Medicine, University of California, Los Angeles
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Chapters in this video
0:00
Introduction
1:11
Fallopian Tube Collection and Preparation for Epithelial Cell Isolation
2:06
Epithelial Cell Isolation and Dissociation
4:18
Digestion of Isolated Epithelial Cells to Obtain a Single-Cell Suspension
6:02
Representative Results
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