Name: dissection of drosophila ovaries
Uploaded: 2006-10-19T00:00:00
Description: Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

<p class="jove_title">Protocol for Drosophila ovary dissection</p>
<ol>
<li>Feed flies with yeast 1-2 days prior to dissecting them to fatten up the ovaries.</li>
<li>Anaesthetize flies using carbon dioxide stream.</li>
<li>Using a pair of tweezers, select a female fly.</li>
<li>Submerge the female fly into 1X PBS.</li>
<li>Grab the fly at its lower thorax with a pair of tweezers.</li>
<li>Tug gently at the lower abdomen with another pair of tweezers until the internal organs in the abdomen are exposed.</li>
<li>Look for the pair of ovaries and...

<ol>
	<li>Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+fragile+X+protein+functions+as+a+negative+regulator+in+the+orb+autoregulatory+pathway.">The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.</a> <em style='font-style: italic'>Dev Cell</em>. <b>8</b>, 331-342 (2005).</li><li>Tan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+autoregulatory+feedback+loop+directs+the+localized+expression+of+the+Drosophila+CPEB+protein+Orb+in+the+developing+oocyte.">An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.</a> <em style='font-style: italic'>Development</em>. <b>128</b>, 1159-1169 (2001).</li></ol>

<p class="jove_content">Here we demonstrate a method of dissecting ovaries in Drosophila.  As the method of culturing the different cell types that exist in the Drosophila ovary has yet been defined, this is a rapid method to obtain the Drosophila ovarian tissue. Essentially, we show that dissecting Drosophila ovaries involves a simple two- or three-step procedure. Subsequent treatment of these dissected ovaries depend on the downstream experiments that will be performed.</p>

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
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<td>Forceps</td>
<td>Tool</td>
<td>Sigma</td>
<td>T43537</td>
<td>&nbsp;</td>
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<td>Glass dissecting dish</td>
<td>Tool</td>
<td>Pyrex</td>
<td> 7220-85</td>
<td>&nbsp;</td>		</tbody>
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dissection of drosophila ovaries

Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

Dissection of Drosophila Ovaries

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