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Preparing Individual Drosophila Egg Chambers for Live Imaging

DOI :

10.3791/3679-v

10:38 min

February 27th, 2012

February 27th, 2012

18,705 Views

1Department of Biochemistry, University of Oxford

The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.

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Drosophila

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