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Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

DOI :

10.3791/55519-v

April 14th, 2017

April 14th, 2017

10,212 Views

1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 3Faculty of Life and Environmental Sciences, University of Tsukuba

We describe a protocol for dissection, fixation, and immunostaining of steroidogenic organs in Drosophila larvae and adult females to study steroid hormone biosynthesis and its regulatory mechanism. In addition to steroidogenic organs, we visualize the innervation of steroidogenic organs as well as steroidogenic target cells such as germline stem cells.

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