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Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

20.0K Views

10:57 min

December 16th, 2014

DOI :

10.3791/52223-v

December 16th, 2014


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Intestinal Stem Cells

Chapters in this video

0:05

Title

1:31

Preparation of the Gastrointestinal Tract and Dissection of the Midgut

3:14

Digestion of the Midgut Tissue to Harvest Cells for FAC Sorting

5:25

FACS to Isolate Intestinal Stem Cells (ISC)

8:52

Results: Representative Analyses of the Sorted Cells

10:17

Conclusion

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