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Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

DOI :

10.3791/52223-v

10:57 min

December 16th, 2014

December 16th, 2014

19,586 Views

1Institut für Biochemie und Molekulare Biologie, Universität Ulm, 2Institut für Immunologie, Universitätsklinikum Ulm

Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

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Intestinal Stem Cells

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