Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

8.7K Views

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07:40 min

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July 31st, 2015

DOI :

10.3791/52384-v

July 31st, 2015

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Keiji Itaka¹, Satoshi Uchida¹, Akitsugu Matsui¹, Kayoko Yanagihara¹, Masaru Ikegami¹, Taisuke Endo², Takehiko Ishii³, Kazunori Kataoka²

¹Graduate School of Medicine, Laboratory of Clinical Biotechnology, The University of Tokyo, ²Graduate School of Engineering, Department of Materials Engineering, The University of Tokyo, ³Graduate School of Engineering, Department of Bioengineering, The University of Tokyo

Summary

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Keywords Gene Transfection

Spheroid Cells

Micropatterned Culture Plates

Genetically modified Cell Transplantation

Thermosensitive Polymer

Polyethylene Glycol PEG Matrix

Polyplex Nanomicelle

Primary Hepatocyte Spheroids

Luciferase

Albumin Expression

Chapters in this video

0:05

Title

1:15

Preparation of 3D Cell Spheroids and Polyplex Nanomicelles

3:33

Gene Transfection and Transplantation of Spheroids and Evaluation of Transgene Expression

5:38

Results: Genetically-modified Cell Transplantation Using Gene Transfected Spheroids

7:14

Conclusion

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