March 25th, 2015
•Here, a method that enables quick, efficient, and inexpensive preparation of polyacrylamide gels in a multiwell plate format is described. The method does not require any specialized equipment and could be easily adopted by any research laboratory. It would be particularly useful in research focused on understanding stiffness-dependent cell responses.
Tags
Related Videos
Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved