July 3rd, 2018
•Substrates with stiffness in the kilopascal-range are useful to study the response of cells to physiologically relevant micro-environment stiffness. Using just a widefield fluorescence microscope, the Young's modulus of soft silicone gels can be determined using an indentation with a suitable sphere.
Related Videos
Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
Simple Polyacrylamide-based Multiwell Stiffness Assay for the Study of Stiffness-dependent Cell Responses
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved