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Preparation of Complaint Matrices for Quantifying Cellular Contraction

DOI :

10.3791/2173-v

December 14th, 2010

December 14th, 2010

17,509 Views

1Institute for Biophysical Dynamics, University of Chicago, 2Physics Department - James Franck Institute, University of Chicago, 3Interdisciplinary Scientist Training Program, University of Chicago

In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

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Complaint Matrices

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