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Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

DOI :

10.3791/52654-v

14:29 min

January 31st, 2015

January 31st, 2015

10,193 Views

1Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory

We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.

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Tissue Repair

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