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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

DOI :

10.3791/1129-v

February 16th, 2009

February 16th, 2009

13,898 Views

1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, 2Departments of Neurology and Neurobiology, University of California, Los Angeles

Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.

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Two photon Axotomy

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