Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

24.2K Views

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11:15 min

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September 20th, 2016

DOI :

10.3791/52758-v

September 20th, 2016

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Alan J. Fox¹, Matthew C. Hiemenz¹, David B. Lieberman¹, Shrey Sukhadia¹, Barnett Li¹, Joseph Grubb¹, Patrick Candrea¹, Karthik Ganapathy¹, Jianhua Zhao¹, David Roth¹, Evan Alley²^,³, Alison Loren²^,³, Jennifer J. D. Morrissette¹

¹Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, ²Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, ³Abramson Cancer Center

Summary

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Keywords Next Generation Sequencing

Targeted Amplicon Assay

Response To Targeted Therapy

Chapters in this video

0:05

Title

1:19

Genomic DNA Quality Control and Amplicon Library Preparation

3:23

Indexing and PCR Amplification

4:37

PCR Purification and Bead-based Normalization

6:33

Sequencing and Data Analysis

7:19

Results: Mutations Detected in Solid and Liquid Tumors Using Next Generation Sequencing

9:06

Conclusion

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