JoVE Logo
Faculty Resource Center

Sign In

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

DOI :

10.3791/61449-v

September 9th, 2020

September 9th, 2020

10,397 Views

1Cancer Genomics and BioComputing of Complex Diseases laboratory, The Azrieli Faculty of Medicine, Bar-Ilan University, 2The Data Science Institute, Bar-Ilan University, 3The Dangoor Centre For Personalized Medicine, Bar-Ilan University

In this paper we present a detailed protocol for non-invasive liquid biopsy technique, including blood collection, plasma and buffy coat separation, cfDNA and germline DNA extraction, quantification of cfDNA or germline DNA, and cfDNA fragment enrichment analysis.

Tags

Cell free DNA

-- Views

Related Videos

article

Cell-Free DNA Integrity Analysis in Urine Samples

article

Serum and Plasma Copy Number Detection Using Real-time PCR

article

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

article

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

article

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

article

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

article

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

article

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

article

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

article

Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved