June 18th, 2015
•Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
Related Videos
Transfecting Human Neural Stem Cells with the Amaxa Nucleofector
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved