JoVE Journal
Biology
A subscription to JoVE is required to view this content.
9.8K Views
•
11:24 min
•
March 25th, 2015
DOI :
March 25th, 2015
•Chapters in this video
0:05
Title
1:23
Cloning Emerald GFP into an Expression Vector Containing Two-finger ZiF Domains
4:22
Purification of the ZiF-EmGFP Fusion Protein
7:17
Protein Transduction of HeLa Cells
10:07
Results: Fusion of 2F-ZiF Domains to EmGFP Enables Delivery of EmGFP to HeLa Cells
10:57
Conclusion
Related Videos
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
5.2K Views
Trypsinizing and Subculturing Mammalian Cells
20.5K Views
Engineering Cell-permeable Protein
14.3K Views
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
16.4K Views
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
25.2K Views
siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
11.3K Views
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
31.3K Views
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
10.7K Views
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
9.1K Views
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
7.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.