JoVE Logo
Faculty Resource Center

Sign In

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

DOI :

10.3791/53175-v

September 2nd, 2015

September 2nd, 2015

10,114 Views

1Biochemistry and Biophysics, Texas A&M University

We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.

Tags

Protein Delivery

-- Views

Related Videos

article

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

article

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

article

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

article

Nanomanipulation of Single RNA Molecules by Optical Tweezers

article

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

article

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

article

Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

article

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

article

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

article

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved