Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
12.1K Views
•
15:57 min
•
July 11th, 2015
DOI :
July 11th, 2015
•Mayara F. Maggioli1,2, Mitchell V. Palmer1, H. Martin Vordermeier3, Adam O. Whelan3, James M. Fosse4, Brian J. Nonnecke1, W. Ray Waters1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture
Chapters in this video
0:00
Title
1:40
Methods
2:05
Long-term Culture
3:38
Elispot Assay Day 1
4:30
Elispot Assay Day 2
5:15
Adherent Cell Isolation
5:37
Flow Cytometry Plate Preparation
6:15
Harvest the 13 Day Long-term Cells
9:05
Elispot Assay Day 3
10:04
Alkaline Phosphatase Preparation
11:08
Substrate Solution Preparation
11:55
Reading the Plate
12:12
Performing Flow Cytometry
12:25
Results
14:37
Conclusions
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